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HI-V 4th Gen Test Kit (Serum / Plasma/ Whole Blood)
Human immunodeficiency virus type I and type II (HI-V-1 and HI-V-2) are enveloped single strain RNA positive viruses.
-1 has been isolated from patients with AIDS and AIDS-related complex, and from healthy individuals with a high risk for developing AIDS.
2 has been isolated from West African AIDS patients and from sero-positive asymptomatic individuals.
The two types of have significant variation in sequences.
HI-V-1 has been divided into three groups: group M (for major), including at least ten subtypes (A through J); group O (for outlier); and group N (for non-M, non-O).
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The HI-V 4th gen test kit is a lateral flow chromatographic immunoassay.
The test cassette consists of:
A burgundy colored conjugate pad containing recombinant HI-V-1+2 antigen conjugated with colloid gold
Anti-P24 antibody conjugated with colloid gold (anti-P24 antibody conjugates),
Rabbit IgG-gold conjugates,
A nitrocellulose membrane strip containing:
Two test bands (HI-V antibody T and antigen T bands)
Two control bands (C band).
(The image includes: 1 drop of serum/plasma/whole blood → 2 drops buffer → 15 minutes)
Bring specimen and test components to room temperature. Mix if thawed.
Open pouch, remove device, and place on flat surface.
Label device with specimen ID.
Add 1 drop of sample (~30 µL) to each of the two sample wells. Then add 2 drops of buffer (~80 µL) to each.
Start timer.
Read results at 15 minutes.
Do not read after 15 minutes.
Positive results can be visible in as short as 1 minute.
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