The gameplay in Sega Superstars Tennis is similar to Sega's Virtua Tennis series, with characters able to perform techniques such as lob shots and drop shots. Characters each have different statistics and proficiency in specific areas, such as power or control. Each character also has their own unique "Superstar State", a special ability that can be activated once an accompanying meter is filled. The effects of a Superstar State vary depending on the character; for example, Sonic transforms to Super Sonic and causes the ball to zig-zag when hit, while Gilius summons thunderbolts that can temporarily stun opponents. The game features 10 courts themed after various Sega games, such as Green Hill Zone from Sonic the Hedgehog and Shibuya Downtown from Jet Set Radio.[3]

Sega Superstars Tennis features several gameplay modes. Superstars mode allows the player to complete various missions themed around a specific Sega games, which take the form of exhibition matches, tournaments and minigame score challenges; completing these missions will unlock new areas based on other games with additional sets of missions to complete. Players will unlock new additional characters, courts, and music for use in gameplay as they progress through Superstars. Match mode offers standard tennis gameplay in singles or doubles