HBsAg Rapid Test Cassette (Serum/Plasma) is a rapid chromatographic immunoassay for
the qualitative detection of Hepatitis B Surface Antigen.
Allow test cassette, serum or plasma specimen to equilibrate to room temperature (15-30°C)
prior to testing.
1. Bring the pouch to room temperature before opening it. Remove the test cassette from the
sealed pouch and use it as soon as possible. Best results will be obtained if the assay is
performed within one hour.
2. Place the test Cassette on a non-absorbent flat surface. With arrows pointing toward the serum
or plasma specimen, add two drops of specimen vertically to the sample pad under the arrows.
3. Start the timer and wait for the red line(s) to appear. The result should be read at 10 minutes.
Note: A low HBsAg concentration might result in a weak line appearing in the test region (T)
after an extended period of time; therefore, do not interpret the result after 30 minutes.
I
POSITIVE:* Two distinct red lines appear. One line should be in the control region (C) and another
line should be in the test region (T).
*NOTE: The intensity of the red color in the test line region (T) will vary depending on the concentration
of HBsAg present in the specimen. Therefore, any shade of red in the test region (T) should be
considered positive.
NEGATIVE: One red line appears in the control region (C). No apparent red or pink line
appears in the test region (T).
INVALID: Control line fails to appear. Insufficient specimen volume or incorrect procedural
techniques are the most likely reasons for control line failure. Review the procedure and
repeat the test with a new test cassette. If the problem persists, discontinue using the test kit
immediately and contac